The product is best stored in the fridge. It will remain viable for 6 months.

How to Use A Mushroom Liquid Culture Syringe
If you are new to mushroom cultivation or looking to expand your knowledge, understanding how to use a mushroom liquid culture syringe is essential. In this guide, we will walk you through the step-by-step process of using a mushroom liquid culture syringe.

Step 1: Gather Your Supplies
Sterilized Substrate to Inoculate (Bag, Jar, Petri)
Spray Bottle of 70% isopropyl alcohol
Roll of Scotch Tape
Nitrile Gloves
Paper towel
Sterile Needle

Step 2: Prepare the Syringe
Before using the liquid culture syringe, gently shake it for 1 minute to evenly distribute the mycelium throughout the liquid. This will ensure a more consistent inoculation.

Step 3: Prepare Your Work Area
Creating a sterile work area is crucial to prevent contamination during the inoculation process. Use a clean and sanitized workspace, preferably in a laminar flow hood or a still air box.

Step 4: Disinfect the Inoculation Site
If you are using an agar plate, wipe the surface with an alcohol wipe to sterilize it. If you are inoculating a grain jar, make sure it has been properly sterilized beforehand. Make sure to disinfect all surfaces and tools with 70% isopropyl alcohol.

Step 5: Inoculate the Inoculation Site
Remove the cap from the liquid culture syringe and carefully insert the needle into the inoculation site. Slowly depress the plunger to release a small amount of liquid culture onto the site. Be cautious not to flood the area, as excessive moisture can lead to contamination.
Scotch Tape Secret: Bags without injection ports can be inoculated through the plastic. The hole can be covered with clean Scotch Tape. Always remove the exposed piece of scotch tape on the dispenser/roll when entering the hood. Scotch Tape comes off the roll sterile due to the way the glue is handled and the way it is manufactured.

Step 6: Repeat the Process
If you have multiple inoculation sites, repeat the previous step for each site. Remember to sterilize the needle with a flame between each inoculation to prevent cross-contamination.

Step 7: Store the Syringe
After you have finished using the liquid culture syringe, replace the cap and store it in a cool and dark place. It is best to store mushroom liquid culture in the fridge. Never freeze liquid culture store it above 95F. This will help preserve the viability of the mycelium for future use.